Two-temperature LATE-PCR endpoint genotyping
نویسندگان
چکیده
منابع مشابه
Two-temperature LATE-PCR endpoint genotyping
BACKGROUND In conventional PCR, total amplicon yield becomes independent of starting template number as amplification reaches plateau and varies significantly among replicate reactions. This paper describes a strategy for reconfiguring PCR so that the signal intensity of a single fluorescent detection probe after PCR thermal cycling reflects genomic composition. The resulting method corrects fo...
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ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2006
ISSN: 1472-6750
DOI: 10.1186/1472-6750-6-44